Suspension Cell Culture from Single Cells
For cell growth monitoring from single cell, limiting dilution or sorting with cell sorter is well known method to generate single cell state in microwell plate. However, many microplates and medium is required to analyze single cell growth, and it is laborious to check if each well contains single cell or not. Also it takes time for imaging of many microplates. Suspension cells are floating in the culture medium and roaming freely, so it is difficult to track growth from single cell.
SIEVEWELL, high density cell arraying device, is suitable for growth monitoring from single cell. Suspension cells are
captured in each nanowell during proliferation.
Monitoring of cell growth from a single cell clarify that even cell line is a mixture of different growth rate of cells. K562 cells are a mixture of cells with different growth rates, 18 hours, 26 hours and 38 hours.
K562 cells (stained with CellBrite Green) were cultured in SIEVEWELL Slide 50 μm. Images were taken every 2 hours with IncuCyte S3 (10x objective lens). Images of day 0 and day 2 were ovelayed using ImageJ.
