デバイス内での培養

シングルセルとなるように分散した細胞を播種することで、ナノウェルの格納されたシングルセルの増殖等を観察可能です。

CellBrigh Green染色したK562細胞を SIEVEWELL Slide 50 μmタイプへロードし、IncuCyte® S3 システムにより2時間インターバルで経時的に撮影を行った。分裂速度の異なる3種類の細胞が混在している。

Features

Imaging

Imaging with microscope

SIEVEWELL Slide is standard glass slide format and compatible with conventional microscope.

Features

イメージング

顕微鏡でのイメージング

SIEVEWELL Slide は標準的なスライドガラスと同サイズです。専用のアダプター等を必要とせず、一般的なチャンバースライドのようにそのまま顕微鏡でのイメージングが可能です。

Applications

On chip CTC staining, detection

…

Applications

CTC Detection from CDX/PDX-Tumor Dearing Mouse

…

Applications

Live cell imaging

Real-time imaging to quantify NETosis with SIEVEWELL

HL-60 cells were cultured for 3 days with 1.3% DMSO to induce differentiation. Neutrophil-differentiated HL-60 cells were incubated for 5 minutes with the membrane-permeable NUCLEAR-ID Red DNA dye to stain nuclei. After three times of washing, NUCLEAR-ID Red stained-neutrophils were loaded into SIEVEWELL. RPMI containing Sytox Green (0.2 µM) and 100 ng/mL PMA was added to induce NETosis and to assess for cell death. SIEVEWELL was placed on IncuCyte® S3 System which is housed inside a cell incubator at 37°C with 5% CO₂. Neutrophils were imaged using phase contrast, red (800 ms exposure) and green (400 ms exposure) channels. Images using a 20x dry objective lens were taken every 5 minutes.

Reference of reagents, scan conditions. J Immunol January 15, 2018, 200 (2) 869-879.