3-1

Aspirate all buffer.

3-2

Add reagent.

3-3

Aspirate 0.2 mL.

Incubate at required temperature for required time

3-4

Add buffer.

3-5

Aspirate

Repeat step 3-4 and 3-5 two times.

3-6

Add 1 mL of PBS.

2-1

Aspirate PBS from side ports.

2-2

Add cell suspension.

2-3

Aspirate 1 mL of buffer.

2-4

Add 1 mL of PBS.

Now ready for next step.

1-1

Add 0.5 mL of Ethanol, then add 0.5 mL of PBS.

1-2

Add 2 mL of PBS.

1-3

Aspirate 2 mL of PBS.

Repeat 1-2 and 1-3 two times.

1-4

Add 1 mL of PBS.

Now ready for cell loading.